Held June 5 and 6, 2000
| Round | Pick | Player | Pos. | School | Hometown | Signed |
|---|
| 1 | 14 | Beau Hale | RHP | Texas | Mauriceville, TX | 8/18/00 |
| 1 (S) | 32 | Nelson Johnson | 3B | Newport, HS | Bellevue, WA | 6/27/00 |
| 3 | 84 | Richard Bartlett | RHP | Kamiakin HS | Kennewick, WA | 6/27/00 |
| 3 | 86 | Thomas Arko | C | Cooper HS | Abilene, TX | 6/27/00 |
| 4 | 114 | Jon Skaggs | RHP | Rice University | Sugarland, TX | |
| 5 | 144 | Douglas Gredvig | 1B | Sacramento CC | Roseville, CA | 6/27/00 |
| 6 | 174 | Brandon Spillers | RHP | Tatnall Square Academy | Roberta, GA | |
| 7 | 204 | B.J. Litleton | OF | Lamar | TX | 6/27/00 |
| 8 | 234 | Jaime Sperring | RHP | Rice University | Sugarland, TX | 6/27/00 |
| 9 | 264 | Mike Russell | C | Bothell HS | Bothell, WA | 6/27/00 |
| 10 | 294 | Casey Cahill | RHP | Immaculata HS | New Brunswick, NJ | |
| 11 | 324 | Darren Heal | RHP | Oklahoma State | | |
| 12 | 354 | Kris Wilken | 3B | Houston | | 6/27/00 |
| 13 | 384 | Ryan Keefer | RHP | Southern Columbia Area HS | Catawissa, PA | 6/27/00 |
| 14 | 414 | Brian Forystek | LHP | Illinois State | | 6/27/00 |
| 15 | 444 | Thomas Joyce | OF | First Presbyterian Day HS | Macon, GA | 6/27/00 |
| 16 | 474 | D.J. Jones | LHP | Texas | | |
| 17 | 504 | Joel Crump | LHP | Yuma HS | Yuma, AZ | 6/27/00 |
| 18 | 534 | Kyle Sleeth | RHP | Northglenn HS | Westminster, CO | N |
| 19 | 564 | Shayne Ridley | SS | Ball State | | 6/27/00 |
| 20 | 594 | Ryan Mask | RHP | Garces Memorial HS | Bakersfield, CA | |
| 21 | 624 | Fraser Dizard | LHP | Meadowdale HS | Edmonds, WA | |
| 22 | 654 | Aaron Bouie | RHP | St. Bonaventure | | 6/27/00 |
| 23 | 684 | Eric Walsh | RHP | El Toro HS | Mission Viejo, CA | |
| 24 | 714 | Jose Rodriguez | SS | Iowa Western CC | | 6/27/00 |
| 25 | 744 | Jeff Petersen | RHP | Kentwood HS | Covington, WA | |
| 26 | 774 | Dan Marchetti | RHP | Ohio Wesleyan | | 6/27/00 |
| 27 | 804 | Scott Koffman | RHP | Olympus HS | Salt Lake City, UT | |
| 28 | 834 | Robert Cheatwood | LHP | Allan Hancock (Calif.) JC | | |
| 29 | 864 | David Morrow | RHP | Sherman (Texas) HS | | |
| 30 | 894 | Donnie Poles | OF | Harding HS | Charlotte, NC | |
| 31 | 924 | Andy Hargrove | 1B | St. Ignatius HS | Cleveland, OH | |
| 32 | 954 | Brandon Fahey | 3B | Grayson County (Texas) CC | | |
| 33 | 984 | Kurt Birkins | OF | Los Angeles Pierce JC | | 6/5/01 |
| 34 | 1014 | Joshua Banks | RHP | Severna Park HS | Arnold, MD | N |
| 35 | 1044 | Jeff Salazar | OF | Connors State (Okla.) JC | | |
| 36 | 1074 | Tim Stauffer | RHP | Saratoga Central Catholic HS | Saratoga Springs, NY | |
| 37 | 1104 | Ryan Newman | SS | Scottsdale CC | Scottsdale, AZ | |
| 38 | 1134 | Chaz Macklin | C | East St. Louis HS | East St. Louis, IL | |
| 39 | 1164 | Matt Harter | C | Penns Valley Area HS | Springs Mill, PA | |
| 40 | 1194 | Sean Patrick | C | John Logan (Ill.) CC | | |
| 41 | 1224 | Justin Ruchti | C | San Jacinto (Texas) JC | | |
| 42 | 1254 | Eric Fagan | LHP | Taft (Calif.) JC | | |
